Reporter

Part:BBa_J100521:Design

Designed by: Maddie Hunter   Group: Campbell M Lab   (2020-01-27)


Experimental Plasmid for Epigenetic Hunter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 7
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1857
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 7
    Illegal AgeI site found at 74
    Illegal AgeI site found at 1287
    Illegal AgeI site found at 1399
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1576
    Illegal BsaI.rc site found at 501


Design Notes

Two BsaI sites used for Golden Gate Assembly to remove RFP and insert promoter of interest


Source

Assembled from PCR products using WT yeast genomic DNA as the template

References